Wax in waxy crude oil is one of the main reasons for the poor fluidity of crude oil. In order to solve the great difficulties brought by the fluidity of waxy crude oil to crude oil mining and gathering, it is necessary to carry out wax removal treatment on crude oil.Microbial paraffin removal technology is the use of microbial metabolism to directly degrade wax components in crude oil and degrade long chain hydrocarbons into medium and short chains, thus reducing the wax content in crude oil at all. By detecting the wax content in crude oil and wax crystal morphology before and after the treatment of compound bacteria, the effects of the compound bacteria on the degradation of wax components in crude oil and the morphology and size of wax crystals in crude oil are studied, it is concluded that the wax removal characteristics of the compound bacteria are analyzed macroscopically. Finally, the paraffin removal effect of the compound bacteria is verified from the microscopic point by detecting the particle size distribution of the oil sample after paraffin removal treatment.